KU Sequencing Lab

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Samples are processed on a first come, first serve basis. Notify staff 24 hours in advance of dropping off or canceling runs. You will be billed if failure to notify under 24 hours. A minimum of 12 Licor (gel slab) samples and 8 Beckman (CE) samples are needed to run the respective instrument. Samples will be held until the minimum number is reached for each system, with delays likely. To avoid delays, you can pay for the remaining samples in order to process your samples faster. See Pricing for electrophoresis rates.

Submitting samples:  Please submit purified (Qiagen column ideal), quantified template, and unlabeled primer (for CE samples). For electrophoresis only, please drop off between 1:00-2:00 pm. Samples needing the sequencing reaction and clean-up procedures should be dropped off by 10:00 am. Amount of template to submit depends on chemistry and size of insert or PCR product (see chart below).

Billing will go out the end of each month.


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Minimum amount of sample to submit by chemistry.


Beckman
template
Licor
template

plasmid 3-6 kb 100-390 ng plasmid 3-10 kb
500-800 ng

plasmid 8-12 kb 260-780 ng
plasmid 10-20 kb
1.0 ug

PCR products 6.5-65 ng M13 ssDNA 200 ng

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